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What are Blank, Control, Standard and Test in research?

Updated: Nov 9, 2023



This is one of the most common and perplexing question in research. Perplexing because many don't figure out the need for a standard or the difference between a blank and a control.


Imagine you working on a plant extract that shows anticancerous property

Can you work without the 'particular' plant extract?

- That is where the test comes in!

How can you say that the plant extract contains potential substance with anticancerous property?

- That is where the control comes in!

How can you say that this substance has great anticancerous potential?

- That is where the standard comes in!

How can you be sure that this is even a plant extract?

- That is where the blank comes in!



In many of the above questions a definite answer is obtained only when we do a comparative between blank, control, standard and test. All these can be broadly classified under "samples" which are a set of factors that is required to prove the research objective.


  • Blank is anything without the special ingredient you want to study

  • Control is anything which contains a particular amount of the special ingredient you want to study

  • Standard is anything which contains the special ingredient in a know quantity

  • Test is anything that contain the special ingredient but the quantity is unknown

Comparing the result of :

  • Test and Standard help in calculating the quantity of special ingredient in the test

  • Test and Control confirms the identity of the special ingredient

  • Test and Control confirms the presence of the special ingredient


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